N100 bp dna ladder promega pdf

The 100 bp dna ladder contains 12 discrete dna fragments ranging from 100 bp to 3,000 bp. The 100 bp dna ladder consists of double stranded molecular weight markers ranging in sizes from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000 bp. The stepup 100 bp dna ladder contains 10 bands of double stranded dna fragments ranging from 100 to bp with size increment of 100 bp. The 100 bp dna ladder is a unique combination of a number. The dna molecular weight marker can be used in conjunction with dna molecular weight marker xiii 50 bp ladder. Description the dna ladders are ladders with defined sizes. Agarose gel analysis shows that the bands between 100 bp and 1,500 bp. The 100 bp dna ladder is a readytoload molecular weight marker suitable for dna fragment size determination on gel electrophoresis. Promega, 100bp dna ladder, 250ul 50 lanes quantity. The dna includes fragments ranging from 100 3,000 base pairs. The 500bp fragment is present at increased intensity for easy identification.

The benchtop dna markers are supplied in a stabilizing solution of 1x blueorange loading dye, which circumvents any requirements for further manipulation. Hyperladder 100bp is a molecular weight marker, composed of a restriction digest plus one or more pcr products, especially designed for easy size determination of linear doublestranded dna fragments on 1% to 2% agarose gels. For instance, the price of 100 bp dna ladder for 50 runs is listed 110 usd g2101 promega and 53 usd n3231snew england biolabs on available web sites. Electrophoreses of dna ladder benchtop 100bp dna ladder. Promega 100bp dna ladder molecular weight marker 100 bp dna ladder. Promega, 100bp dna ladder, 250ul 50 lanes proficient lab. Promega 100bp dna ladder molecular weight marker fisher. Generuler 100 bp plus dna ladder thermo fisher scientific. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100.

Straightforward procedure for laboratory production of dna. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. Ladder is provided with a tube of 6x blueorange loading dye. The 100bp dna step ladder has 40 bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments. The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of gel loading dye, purple 6x, no sds. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. A generuler 100 bp dna ladder red and promega 100 bp dna ladder blue.

Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. The 500 bp band with higher concentration is distinguished easily from others. The trackit 100 bp dna ladder is formulated with unique tracking dyes, xylene cyanol ff xcff and tartrazine, that allow you to visually track dna. Supplied with free vial of gel loading dye, purple 6x. Readytoload 100 bp dna ladder ranging from 100 to 2,000. Sigmas pcr 100 bp low marker is provided in a solution of 10 mm trishcl ph 8. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an. Two internal features facilitate band identification. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2000 bp.

The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of gel. Promega 100bp dna ladder molecular weight marker 100 bp dna. Thermo scientific generuler 100 bp dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 100 bp to 1,000 bp on agarose or polyacrylamide gels. We estimated that our protocol spends only 3 usd for materials to produce 50 runs of 100 bp dna ladder. Generuler 100 bp dna ladder thermo fisher scientific. Promega 100bp dna step ladder molecular weight marker for electrophoresis forty bluntended dna.

The dna fragments may be stained with ethidium bromide. The ladder is prepared from a plasmid containing repeats of a 100 bp dna fragment. In addition, our strategy is flexible for producing different kinds of dna ladders. Trackit 100 bp dna ladder thermo fisher scientific. The ladders are not intended for use in quantitative analysis. Our fullranger contains sixteen discrete fragments ranging from 100 bp to 5000 bp with higher intensity reference bands at 500 bp and bp. These are suitable for sizing of dna fragmentsgenerated by pcr, restriction digestion, etc. Promega 100bp dna step ladder molecular weight marker for. The approximate mass of dna in each band is provided 0. Straightforward procedure for laboratory production of dna ladder. The fragments may be stained with ethidium bromide. If samples need to be diluted, use te or other buffer of minimal ionic strength. L 11 fragments from 100bp to 1,000bp in 100bp increments and includes an additional 1. Promega 100bp dna step ladder molecular weight marker for electrophoresis forty bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments.

Dna molecular weight marker xiv 100 bp ladder sigma. The digested dna includes fragments ranging from 100 bp to 1,517 bp. Dna molecular weight marker xiv 100 bp ladder allows accurate sizing of dna fragments generated by pcr or restriction digest or rtpcr, following separation on agarose gels. The 100 bp dna ladder is formulated to run accurately and to provide crisp band patterns. Thermo scientific generuler 100 bp dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 100 bp to 1,000 bp on agarose or. The ladder is not intended for use in quantitative analysis. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. Other notes contains 10 bands, 100 1,000 bp in exact 100 bp spaced ladder recombinant repeats.

Promega 100bp dna ladder molecular weight marker 100 bp. Promega manufacturing and delivery systems continue to be fully operational during the covid19. A highintensity 500bp band stands out at the lowest segment of the ladder 1kb. The 100 bp ladder marker bp from geneon is designed for use as molecular weight standard for agarose gel electrophoresis. B generuler 100 bp dna ladder red and directload pcr 100 bp low ladder blue. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. Each ladder is provided with a tube of 6x blueorange loading dye. The readytouse 100bp ladder, premixed with loading buffer dye, ranges from 1,500 to 100 bp and has intense reference bands at 1500bp and 500 bp. It contains bromophenol blue dye which serves as visual aid to monitor the progress of migration during agarose gel electrophoresis. The 10 regularly spaced bands, ranging from 100 bp to 10 bp, with a doublet band at 300311 bp. Pcr 100 bp low ladder for electrophoresis of pcr fragments. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp.